ADC开发解决方案

抗体偶联药物( antibody-drug conjugate,ADC) 是将单克隆抗体药物的高特异性和小分子细胞毒药物的高活性相结合,用以提高肿瘤药物的靶向性、减少毒副作用。ADC抗体分子的筛选是ADC临床转化前的重要基础,是保证ADC临床有效性和安全性的关键。

澳门新莆京9767针对每个ADC热门靶点,已经开发出几十种预先开发好的先导分子,同时建立了一整套ADC临床前药效学评价平台。

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ADC 开发流程

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5000+ 现货先导抗体分子
覆盖400+ 药靶

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人/猴交叉反应评估

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亲和力排序

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体外肿瘤细胞抑制试验

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体内肿瘤抑制试验

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毒素偶联

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内吞测试

 

案例展示

ADC Cell Killing Assay with MMAE conjugated GPRC5D Antibodies (Ab1 and Ab2)

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Specific Cell Killing by Anti-GPRC5D Antibodies Conjugated with MMAE
In this experiment, three cell types were tested: K562 (negative control, left), K562-GPRC5D (positive cells, center), and H929 (GPRC5D-expressing natural cell line, right). The black and red curves represent MMAE-conjugated anti-GPRC5D antibodies (Ab1/2), showing specific killing of GPRC5D-positive cells. The blue curve represents MMAE-conjugated anti-GPRC5D positive control antibodies, and the green curve represents MMAE-conjugated IgG negative control. The data demonstrates the specific cytotoxicity of anti-GPRC5D antibodies against GPRC5D-positive cells following MMAE conjugation.

Cyno and mouse cross-reactivity tests

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Cross-Reactivity Analysis of GPRC5D Candidate Antibodies with Cyno and Mouse GPRC5D Positive Cells Using FACS

In this study, we assessed four distinct cell types: 293T-hu GPRC5D, 293-cyno GPRC5D, 293-mouse GPRC5D, and 293T-negative control. The negative control, 293-PTT5 cells, lacked overexpressed GPRC5D proteins, while the other cells contained overexpressed GPRC5D proteins from human, cyno, and mouse species. We employed candidate antibodies, including G01~G09 (humanized antibodies developed by DIMA Bio) and G00 (a humanized antibody molecule developed by Eureka Therapeutics), to interact with these four cell types using FACS. Our data clearly reveals that G00, G05, G07, and G09 antibodies exhibit cross-reactivity with Cynomolgus Monkey GPRC5D, while the G00 antibody displays cross-reactivity with Mouse GPRC5D.

DiTag TM IgG Labeling Reagent for Antibody Internailzaiton Assay

Antibody Internalization Assay with DiTag TM Reagents

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Specific Internalization of GPRC5D ADC BMK-AME100001 Conjugate in GPRC5D-Positive Cells (K562-GPRC5D Stable Expression Cell Line, Right), with No Detection in K562 Cells Lacking GPRC5D Overexpression (Left).

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Specific Internalization of GPRC5D ADC BMK-AME100002 Conjugate in GPRC5D-Positive Cells (K562-GPRC5D Stable Expression Cell Line, Right), with No Detection in K562 Cells Lacking GPRC5D Overexpression (Left).

Stability Testing for DiTag TM Reagents

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Stability test of AME100001: Three storage methods are tested: lyophilization and reconstitution (black), liquid with 50% glycerol at -20℃ (red), liquid at 4℃ (blue). All three methods exhibit excellent stability.

Reagents for ADC payload detection

antibody-DME101004 MMAE Fig.1 Elisa 1

Figure1. Elisa plates were pre-coated with IgG-MMAE (0.2μg/per well). Serial diluted anti-MMAE monoclonal antibody (DME101004) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-MMAE monoclonal antibody binding with IgG-MMAE is 1.356ng/ml.

 

ADC开发工具

澳门新莆京9767致力于推动ADC研究,并为研究人员提供所需的工具和技术服务,以便于更好的定义精准医疗。我们邀请您加入我们的变革之旅,共创癌症治疗的未来。

 

平台优势

功能活性重组蛋白

1000+ 重组ECD蛋白 .

200+ 全长多穿膜蛋白

现货先导抗体分子

80+ ADC先导抗体分子 .

400+ 预制B细胞种子库

ADC 开发工具

2 评估内吞效果的IgG标记试剂

5 anti-payload 兔单抗.

成功案例

10+ 已转让ADC项目.

20+ 测试中 ADC 项目.

 

服务流程

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ADC 项目合作

依托强大专业的技术团队,澳门新莆京9767可为客户提供个性化的ADC开发服务,协助客户加快ADC药物研发进程。

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